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Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74E-WPRE
(Plasmid #250392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250392 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti CMV GFP Puro
  • Backbone manufacturer
    Eric Campeau, Paul Kaufman (Addgene #17448)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUDT5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • Mutation
    W28A, Y74E
  • Entrez Gene
    NUDT5 (a.k.a. YSA1, YSA1H, YSAH1, hNUDT5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry-GSG-P2A (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert cloned with BamHI (5') and SalI (3') sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74E-WPRE was a gift from Stefan Kubicek (Addgene plasmid # 250392 ; http://n2t.net/addgene:250392 ; RRID:Addgene_250392)

  • For your References section:

    A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesis. Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl C, Marella N, Buphamalai P, Traversi F, Beres M, Moll HP, Siklos M, Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, Lang K, Huber KVM, Kubicek S. Science. 2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257. 10.1126/science.adv4257 PubMed 41196952
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