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pSBinit-Syb-BC10(Y35A/Y56A/R60A/I61T/H63A/T105A/Y107A/W108S)
(Plasmid #250415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250415 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSBinit
  • Backbone manufacturer
    Markus Seeger (Addgene # 110100)
  • Vector type
    Bacterial Expression, Affinity Reagent/ Antibody

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Syb-BC10
  • Alt name
    Non-Binding Anti-SLC66A2 Sybody
  • Species
    Synthetic
  • Insert Size (bp)
    366
  • Mutation
    CDR1(Y35A)/CDR2(Y56A/R60A/I61T/H63A)/CDR3(T105A/Y107A/W108S)
  • Promoter araBAD
  • Tags / Fusion Proteins
    • PelB (N terminal on backbone)
    • Myc-6xHis (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBinit-Syb-BC10(Y35A/Y56A/R60A/I61T/H63A/T105A/Y107A/W108S) was a gift from Simon Newstead (Addgene plasmid # 250415 ; http://n2t.net/addgene:250415 ; RRID:Addgene_250415)
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