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pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-TEV-GFP-His8
(Plasmid #250427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250427 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLexM
  • Backbone manufacturer
    Edith Yvonne Jones (Addgene # 99844)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PQLC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    702
  • Mutation
    Δ100-125 (Replaced with GGGS), Δ257-271
  • Entrez Gene
    SLC66A2 (a.k.a. PQLC1)
  • Promoter Chicken-beta-actin promoter/CMV Enhancer
  • Tag / Fusion Protein
    • TEV-GFP-8xHis (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer GCTGGTTGTTGTGCTGTCTCATC
  • 3′ sequencing primer ATCACCATCTAATTCAACCAA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-TEV-GFP-His8 was a gift from Simon Newstead (Addgene plasmid # 250427 ; http://n2t.net/addgene:250427 ; RRID:Addgene_250427)
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