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pcDNA3.1(+)_AlphaCARD_ALFA_231232
(Plasmid #250443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250443 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6597
  • Modifications to backbone
    NA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha Cardiac Muscle Actin
  • Alt name
    ACTC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • GenBank ID
    NM_001406482
  • Entrez Gene
    ACTC1 (a.k.a. ACTC, ASD5, CMD1R, CMH11, LVNC4)
  • Promoter CMV Promotor
  • Tag / Fusion Protein
    • ALFA Tag (Internal)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_AlphaCARD_ALFA_231232 was a gift from Koen van den Dries (Addgene plasmid # 250443 ; http://n2t.net/addgene:250443 ; RRID:Addgene_250443)

  • For your References section:

    IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms. van Zwam MC, Dhar A, Bosman W, van Straaten W, Weijers S, Seta E, Joosten B, van Haren J, Palani S, van den Dries K. PLoS Biol. 2024 Mar 11;22(3):e3002551. doi: 10.1371/journal.pbio.3002551. eCollection 2024 Mar. 10.1371/journal.pbio.3002551 PubMed 38466773
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