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pPR-1_14E_LD5N
(Plasmid #250464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250464 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAGM1311
  • Backbone manufacturer
    Sylvestre Marillonnet
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14E_LD5N
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GRASP kit for MoClo assembly of sPPR proteins is a library of 42 plasmids which can be combined to assemble sPPR proteins with 9, 14, or 19 motifs to target any RNA sequence of the same length. See the original publication for more information, and find code for analysis and script for GRASP assembly at https://github.com/farleykvdg/GRASP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPR-1_14E_LD5N was a gift from Ian Small (Addgene plasmid # 250464 ; http://n2t.net/addgene:250464 ; RRID:Addgene_250464)

  • For your References section:

    GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins. Dennis M, Low SY, Viljoen A, Pullakhandam A, Colas des Francs-Small C, Campbell-Clause L, Bond CS, Small I, Kwok van der Giezen FM. Nucleic Acids Res. 2025 Oct 28;53(20):gkaf1169. doi: 10.1093/nar/gkaf1169. 10.1093/nar/gkaf1169 PubMed 41224122
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