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pT2HE_SALR_MYH3C2-C3
(Plasmid #250495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250495 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pT2HE
  • Backbone manufacturer
    Tim Howes
  • Backbone size w/o insert (bp) 6440
  • Total vector size (bp) 9000
  • Vector type
    Gene expression and genomic integration in fish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SALR_MYH3C2-C3_intergenic_region
  • Species
    Gasterosteus aculeatus
  • Insert Size (bp)
    2557

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PspOMI (unknown if destroyed)
  • 3′ cloning site PspOMI (unknown if destroyed)
  • 5′ sequencing primer GCACCTTGACGATGCTGT
  • 3′ sequencing primer CGTACTCATGGTGGAGCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.22.696110 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2HE_SALR_MYH3C2-C3 was a gift from David Kingsley (Addgene plasmid # 250495 ; http://n2t.net/addgene:250495 ; RRID:Addgene_250495)

  • For your References section:

    Rapid and repeated evolution of myosin copy number in threespine stickleback. Yoxsimer AM, Daugherty RR, Hare EE, Chan YF, Jones FC, Kingman GAR, Offenberg EG, Howes TR, Zhang H, Pollen AA, Brady SD, Xie KT, Chen HI, Lowe CB, Au EH, Grimwood J, Schmutz J, Myers RM, Schluter D, Heins DC, Reyes ML, Baker JA, Jónsson B, Reimchen TE, Bell MA, Kingsley DM.. Current Biology (2026) 10.1016/j.cub.2026.02.052
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