pGL2_299
(Plasmid
#250497)
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PurposepTHT15 Origin | Erythromycin Resistance Cassette | Conjugative Vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneCamR | mScarlet | R6K Ori | OriT
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir2
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameermC
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SpeciesSynthetic
- Promoter pErm
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer M13F
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL2_299 was a gift from Nili Ostrov (Addgene plasmid # 250497 ; http://n2t.net/addgene:250497 ; RRID:Addgene_250497) -
For your References section:
A genetic platform for a biocementation bacterium. Ortiz L, Esmurria A, Gilbert C, Crits-Christoph A, Barnum TP, Mancuso CP, Kang SC, Leung J, Fenn K, Abrams MB, Brumwell SL, Lee HH, Ostrov N. bioRxiv 2025.12.05.692644 10.64898/2025.12.05.692644