pGLU_446
(Plasmid
#250501)
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PurposeP12 driving Himar.004 codon harmonized transposase | Barcoded Erythromycin Resistance Cassette Tn Payload | Conjugative Vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneAmpR | R6K Ori | OriT
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Pir2
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Growth instructionsHigh basal transposase activity. Grow in lower copy Pir strain and/or at low temp.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHimar.004
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SpeciesSynthetic
- Promoter P12
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer oCG0036
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGLU_446 was a gift from Nili Ostrov (Addgene plasmid # 250501 ; http://n2t.net/addgene:250501 ; RRID:Addgene_250501) -
For your References section:
A genetic platform for a biocementation bacterium. Ortiz L, Esmurria A, Gilbert C, Crits-Christoph A, Barnum TP, Mancuso CP, Kang SC, Leung J, Fenn K, Abrams MB, Brumwell SL, Lee HH, Ostrov N. bioRxiv 2025.12.05.692644 10.64898/2025.12.05.692644