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pEHE51
(Plasmid #250522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250522 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYDE007
  • Backbone manufacturer
    Edward Geisinger, Addgene plasmid #194152
  • Total vector size (bp) 8854
  • Modifications to backbone
    G239G (GGG > GGA) silent mutation in beta lactamase to remove BsaI recognition site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can use Carbenicillin 50 ug/mL
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold
  • gRNA/shRNA sequence
    TGAGACCGGATACGGTCTCA
  • Species
    Acinetobacter baumannii
  • Promoter J23119

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AGTTGAGACCGGATACGGTCTCAGTTTTAGAGCTAGAAATAGCAAG
  • 3′ sequencing primer AACTGAGACCGTATCCGGTCTCAACTAGTATTATACCTAGGACTGAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.04.574119 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEHE51 was a gift from Ralph Isberg (Addgene plasmid # 250522 ; http://n2t.net/addgene:250522 ; RRID:Addgene_250522)

  • For your References section:

    Dysfunction from loss of essential outer membrane functions causes drug hypersensitization in Acinetobacter baumannii overexpressing multidrug efflux pumps. Hamami E, Huo W, Neal K, Neisewander I, Geisinger E, Isberg RR. bioRxiv [Preprint]. 2025 Aug 4:2024.01.04.574119. doi: 10.1101/2024.01.04.574119. 10.1101/2024.01.04.574119 PubMed 38260615
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