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pET24b-FLAG-Ash0.1
(Plasmid #250523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250523 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET24b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ash0.1
  • Species
    Synthetic
  • Insert Size (bp)
    198
  • Promoter lac1
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • 6xHis tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.29.691301 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET24b-FLAG-Ash0.1 was a gift from Andrew Woolley (Addgene plasmid # 250523 ; http://n2t.net/addgene:250523 ; RRID:Addgene_250523)

  • For your References section:

    An ultra-low background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain. Le GNT, Pham LMT, Xue B, Uppalapati M, Woolley GA. bioRxiv 2025.11.29.691301 10.1101/2025.11.29.691301
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