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P30: FenixS-VP16-NLS-IRES-E-Ash1
(Plasmid #250553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250553 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pKT214 (pSBC-2 based)
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5202
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    FenixS
  • Species
    Synthetic
  • Insert Size (bp)
    438
  • Promoter SV40
  • Tag / Fusion Protein
    • VP16-NLS (C terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SV40pro-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Ash1
  • Species
    Synthetic
  • Insert Size (bp)
    159
  • Promoter SV40
  • Tag / Fusion Protein
    • E protein (N terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.29.691301 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P30: FenixS-VP16-NLS-IRES-E-Ash1 was a gift from Andrew Woolley (Addgene plasmid # 250553 ; http://n2t.net/addgene:250553 ; RRID:Addgene_250553)

  • For your References section:

    An ultra-low background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain. Le GNT, Pham LMT, Xue B, Uppalapati M, Woolley GA. bioRxiv 2025.11.29.691301 10.1101/2025.11.29.691301
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