An ultra-low background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain
Le GNT, Pham LMT, Xue B, Uppalapati M, Woolley GA
bioRxiv 2025.11.29.691301
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250520 | pBAD-FenixS::HO1 | Bacterial expression of the photoswitchable protein FenixS - co-expressed with Heme Oxygenase 1 |
| 250521 | pET24b-FenixS | Bacterial expression of the photoswitchable protein FenixS |
| 250523 | pET24b-FLAG-Ash0.1 | Bacterial expression of the binder Ash0.1 |
| 250525 | pET24b-FLAG-Ash0.8 | Bacterial expression of the binder Ash0.8 |
| 250526 | pET24b-FLAG-Ash0.9 | Bacterial expression of the binder Ash0.9 |
| 250527 | pET24b-FLAG-Ash1 | Bacterial expression of the binder Ash1 |
| 250528 | pTriEx-mCherry-FenixS | Mammalian expression of mCherry-tag FenixS |
| 250529 | pTriEx-NTOM20-mVenus-Ash1 | Mammalian expression of mitochondria-targeted mVenus-tag Ash1 |
| 250530 | P24: pHR-SFFVp-FUSN-mCherry-Ash1 | Mammalian expression of Ash1 tagged with N terminal domain of FUS (1-214) and mCherry |
| 250531 | P25: pHR-SFFVp-NLS-FenixS-EGFP-FTH1 | Self-assebled 24-mer tagged with NLS, FenixS, and EGFP. Upon red-light activation can bind up to 24 Ash1-modified proteins. |
| 250553 | P30: FenixS-VP16-NLS-IRES-E-Ash1 | Mammalian expression of FenixS-VP16-NLS and E-Ash1, optogenetic system for far red-light control transcriptional activation |
| 250554 | P31: FenixS-FUSN-VP16-NLS-IRES-E-Ash1 | Mammalian expression of FenixS-FUSN-VP16-NLS and E-Ash1, optogenetic system for far red-light control transcriptional activation |