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pTriEx-NTOM20-mVenus-Ash1
(Plasmid #250529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250529 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTriEx
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ash1
  • Promoter CMV
  • Tags / Fusion Proteins
    • NTOM20-mVenus (N terminal on backbone)
    • 6xHis tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer aagtatcgggccctttgtgc
  • 3′ sequencing primer GGCAGCCTGCACCTGAGGTTAATCAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.29.691301 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTriEx-NTOM20-mVenus-Ash1 was a gift from Andrew Woolley (Addgene plasmid # 250529 ; http://n2t.net/addgene:250529 ; RRID:Addgene_250529)

  • For your References section:

    An ultra-low background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain. Le GNT, Pham LMT, Xue B, Uppalapati M, Woolley GA. bioRxiv 2025.11.29.691301 10.1101/2025.11.29.691301
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