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pLenti-Cas9-T2A-mNeonGreen-2-bc6
(Plasmid #250524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250524 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
  • Backbone manufacturer
    James Eshleman, Addgene plasmid #211471
  • Backbone size w/o insert (bp) 13723
  • Total vector size (bp) 13736
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    M13F-AATTGGCC
  • Species
    Synthetic
  • Insert Size (bp)
    24

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13F: GTAAAACGACGGCCAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.03.535384 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Cas9-T2A-mNeonGreen-2-bc6 was a gift from James Eshleman (Addgene plasmid # 250524 ; http://n2t.net/addgene:250524 ; RRID:Addgene_250524)

  • For your References section:

    Mechanism of delayed cell death following simultaneous CRISPR-Cas9 targeting in pancreatic cancers. Teh SSK, Halper-Stromberg E, Morsberger L, Bennett A, Bowland K, Skaist A, Cai F, Liang H, Hruban RH, Roberts NJ, Scharpf RB, Zou YS, Eshleman JR. bioRxiv [Preprint]. 2023 Apr 5:2023.04.03.535384. doi: 10.1101/2023.04.03.535384. 10.1101/2023.04.03.535384 PubMed 37066222
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