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pLVX-TetOn-puro-ICP4-∆CTA
(Plasmid #250613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250613 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-TetOn-puro
  • Backbone size w/o insert (bp) 9180
  • Total vector size (bp) 11519
  • Modifications to backbone
    Insertion of codon-optimized HSV-1 ICP4 n208 variant (∆CTA, missing entire C-terminal activation domain) at the MCS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ICP4-n208
  • Alt name
    ∆CTA
  • Alt name
    n208
  • Species
    Herpes simplex virus 1 (KOS strain)
  • Insert Size (bp)
    2325
  • Mutation
    Codon-optimized ICP4 is truncated after first 774 amino acids (missing entire C-terminal Activation Domain)
  • Promoter TRE3GS promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cgtaccacttcctaccctcgtaaagaattcgcaggctcttaaggccaccA
  • 3′ sequencing primer GGGGAGGTGGTCTGGgagggagaggggcatcgatTTATGGCTCCGGTCCAGGTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ICP4-n208 (∆CTA) fragment was cloned from Addgene #250609
Please visit https://doi.org/10.1101/2025.06.20.660750 for our bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOn-puro-ICP4-∆CTA was a gift from Liam Holt (Addgene plasmid # 250613 ; http://n2t.net/addgene:250613 ; RRID:Addgene_250613)
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