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pLVX-TetOn-puro-ICP8
(Plasmid #250616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250616 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX-TetOn-puro
  • Backbone size w/o insert (bp) 9180
  • Total vector size (bp) 12796
  • Modifications to backbone
    Insertion of codon-optimized ICP8 at the MCS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ICP8
  • Alt name
    Infected Cell Protein 8
  • Alt name
    UL29
  • Species
    Herpes simplex virus 1 (KOS strain)
  • Insert Size (bp)
    3591
  • Mutation
    Codon-optimized for Homo sapiens
  • Promoter TRE3GS promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gctcttaaggccaccATGGAGACGAAGCCGAAGA
  • 3′ sequencing primer tccagcttagctcGCAGGGGAGGTGGTCTGGATC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Codon-optimized HSV-1 ICP8 gene block was ordered from Twist Bioscience

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.20.660750 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-TetOn-puro-ICP8 was a gift from Liam Holt (Addgene plasmid # 250616 ; http://n2t.net/addgene:250616 ; RRID:Addgene_250616)
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