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pUBC-SV40NLS-HoTag3-Shunzhi12nm-TSapphire
(Plasmid #250617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250617 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    lentiviral vector
  • Backbone size w/o insert (bp) 7461
  • Total vector size (bp) 8544
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    12nm Shunzhi 60mers
  • Alt name
    PDB 8f53
  • Alt name
    Shunzhi-60mer
  • Species
    Synthetic
  • Insert Size (bp)
    162
  • Promoter pUBC
  • Tags / Fusion Proteins
    • 4X(GGGGS) linker (C terminal on insert)
    • TSapphire (C terminal on insert)
    • HOTag3 (N terminal on insert)
    • SV40NLS (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthetic construct for 12nm-nucGEMs originally cloned from PDB 8f53, associated with DOI: 10.1126/science.adf6591

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.20.660750 for our bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUBC-SV40NLS-HoTag3-Shunzhi12nm-TSapphire was a gift from Liam Holt (Addgene plasmid # 250617 ; http://n2t.net/addgene:250617 ; RRID:Addgene_250617)
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