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pGLU_390
(Plasmid #250625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250625 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    AmpR | R6K Ori | OriT
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Pir2
  • Growth instructions
    High basal transposase activity due to strong promoter in the sfGFP dropout part. Grow in a low copy Pir strain (Pir2, etc) and/or at lower temp.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tn5.003
  • Species
    Synthetic

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLU_390 was a gift from Nili Ostrov (Addgene plasmid # 250625 ; http://n2t.net/addgene:250625 ; RRID:Addgene_250625)

  • For your References section:

    A genetic platform for a biocementation bacterium. Ortiz L, Esmurria A, Gilbert C, Crits-Christoph A, Barnum TP, Mancuso CP, Kang SC, Leung J, Fenn K, Abrams MB, Brumwell SL, Lee HH, Ostrov N. bioRxiv 2025.12.05.692644 10.64898/2025.12.05.692644
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