pESUMO-RsmB-4AAYBBR
(Plasmid
#250650)
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PurposePlasmid for expression of E.coli methyltransferase protein RsmB tagged after 4 amino acids with a YBBR tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepESUMO
- Backbone size w/o insert (bp) 5735
- Total vector size (bp) 7058
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRsmB
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SpeciesE. Coli
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Insert Size (bp)1323
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MutationYBBR tag inserted after the 4th Amino acid from the N terminus of the insert
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Entrez GenersmB (a.k.a. b3289, ECK3275, fmu, fmv, rrmB, sun, yhdB)
- Promoter T7
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Tag
/ Fusion Protein
- YBBR
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pESUMO-RsmB-4AAYBBR was a gift from Olivier Duss (Addgene plasmid # 250650 ; http://n2t.net/addgene:250650 ; RRID:Addgene_250650)
Map uploaded by the depositor.
