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pVB240306
(Plasmid #250660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250660 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET
  • Backbone manufacturer
    VectorBuilder
  • Backbone size w/o insert (bp) 5636
  • Total vector size (bp) 6455
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ChREBP alpha GSM domain
  • Alt name
    MetHis6mouseChREBPalpha43-307
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    819
  • Mutation
    contains aa 43-307 only, with optimized E. coli codons
  • Entrez Gene
    Mlxipl (a.k.a. ChREBP, Mlx, WS-bHLH, Wbscr14, bHLHd14)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVB240306 was a gift from Charles Brenner (Addgene plasmid # 250660 ; http://n2t.net/addgene:250660 ; RRID:Addgene_250660)

  • For your References section:

    Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency. Tiwari V, Jin B, Sun O, Lopez Gonzalez EDJ, Chen MH, Wu X, Shah H, Zhang A, Herman MA, Spracklen CN, Goodman RP, Brenner C. Nat Metab. 2025 Nov;7(11):2284-2299. doi: 10.1038/s42255-025-01399-3. Epub 2025 Nov 14. 10.1038/s42255-025-01399-3 PubMed 41238906
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