pAL750 :: msfGFP
(Plasmid
#250661)
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PurposeInducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcanii
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAL750
- Backbone size w/o insert (bp) 8716
- Total vector size (bp) 9430
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Modifications to backbonepAL750 is pTA962 with pXyl promoter added
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Vector typeHaloarchaeal expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMonomeric Superfolder Green Fluorescent Protein
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Alt namemsfGFP
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SpeciesSynthetic
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Insert Size (bp)714
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAL750 :: msfGFP was a gift from Alexandre Bisson (Addgene plasmid # 250661 ; http://n2t.net/addgene:250661 ; RRID:Addgene_250661) -
For your References section:
A sweet new set of inducible and constitutive promoters in Haloferax volcanii. Rados T, Andre K, Cerletti M, Bisson A. Front Microbiol. 2023 Aug 10;14:1204876. doi: 10.3389/fmicb.2023.1204876. eCollection 2023. 10.3389/fmicb.2023.1204876 PubMed 37637112