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pAJS1209 GFP-Tau-160-210-T2A-mApple
(Plasmid #250742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250742 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAJS1197 Cul5-3xFlag-T2A-mApple
  • Backbone manufacturer
    Kampmann Lab
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tau
  • Species
    H. sapiens (human)
  • Mutation
    aa 160-210 only
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)
  • Promoter CAGG
  • Tag / Fusion Protein
    • EmGFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.06.16.545386 for bioRxiv preprint.

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, finding R19Q in mApple. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAJS1209 GFP-Tau-160-210-T2A-mApple was a gift from Martin Kampmann (Addgene plasmid # 250742 ; http://n2t.net/addgene:250742 ; RRID:Addgene_250742)

  • For your References section:

    CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis. Samelson AJ, Ariqat N, McKetney J, Rohanitazangi G, Parra Bravo C, Bose RS, Travaglini KJ, Lam VL, Goodness D, Ta T, Dixon G, Marzette E, Jin J, Tian R, Tse E, Abskharon R, Pan HS, Carroll EC, Lawrence RE, Gestwicki JE, Rexach JE, Eisenberg DS, Kanaan NM, Southworth DR, Gross JD, Gan L, Swaney DL, Kampmann M. Cell. 2026 Jan 28:S0092-8674(25)01487-4. doi: 10.1016/j.cell.2025.12.038. 10.1016/j.cell.2025.12.038 PubMed 41610849
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