pAJS1222 SOCS4-HA-IRES-Puro-T2A-mTagBFP2
(Plasmid
#250756)
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PurposeVector for lentiviral expression of SOCS4-HA with a Puro-T2A-mTAGBFP2 marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250756 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepMK1334
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Backbone manufacturerKampmann Lab
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOCS4
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SpeciesH. sapiens (human)
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Entrez GeneSOCS4 (a.k.a. SOCS7)
- Promoter CAGG
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.06.16.545386 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAJS1222 SOCS4-HA-IRES-Puro-T2A-mTagBFP2 was a gift from Martin Kampmann (Addgene plasmid # 250756 ; http://n2t.net/addgene:250756 ; RRID:Addgene_250756) -
For your References section:
CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis. Samelson AJ, Ariqat N, McKetney J, Rohanitazangi G, Parra Bravo C, Bose RS, Travaglini KJ, Lam VL, Goodness D, Ta T, Dixon G, Marzette E, Jin J, Tian R, Tse E, Abskharon R, Pan HS, Carroll EC, Lawrence RE, Gestwicki JE, Rexach JE, Eisenberg DS, Kanaan NM, Southworth DR, Gross JD, Gan L, Swaney DL, Kampmann M. Cell. 2026 Jan 28:S0092-8674(25)01487-4. doi: 10.1016/j.cell.2025.12.038. 10.1016/j.cell.2025.12.038 PubMed 41610849