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AraC-S170-LOV-C450A-D432V(M392)
(Plasmid #250776)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250776 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTKEI-Dest modified
  • Backbone size w/o insert (bp) 3656
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AraC with an AsLOV2 insertion behind S170 and C450A and D432V mutation
  • Species
    E. coli, Avena sativa
  • Insert Size (bp)
    1338
  • Mutation
    C450A, D432V
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AraC-S170-LOV-C450A-D432V(M392) was a gift from Dominik Niopek (Addgene plasmid # 250776 ; http://n2t.net/addgene:250776 ; RRID:Addgene_250776)

  • For your References section:

    Modular Engineering of Thermo-Responsive Allosteric Proteins. Kroell AS, Hoffmann KH, Motzkus NA, Lemmen N, Happ N, Wolf B, von Bachmann A, Southern N, Vogd F, Aschenbrenner S, Niopek D, Mathony J. Nat Chem Biol (2026). 10.1038/s41589-026-02151-y
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