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AcrIIA5-E76-LOV-C450A-K488N(M478)
(Plasmid #250778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250778 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5407
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrIIA5 with an AsLOV2 insertion behind E76 and C450A and K488N mutation
  • Species
    Streptococcus thermophilus, Avena sativa
  • Insert Size (bp)
    852
  • Mutation
    C450A, K488N

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AcrIIA5-E76-LOV-C450A-K488N(M478) was a gift from Dominik Niopek (Addgene plasmid # 250778 ; http://n2t.net/addgene:250778 ; RRID:Addgene_250778)

  • For your References section:

    Modular Engineering of Thermo-Responsive Allosteric Proteins. Kroell AS, Hoffmann KH, Motzkus NA, Lemmen N, Happ N, Wolf B, von Bachmann A, Southern N, Vogd F, Aschenbrenner S, Niopek D, Mathony J. Nat Chem Biol (2026). 10.1038/s41589-026-02151-y
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