Skip to main content
Addgene
Open Menu

SpyCas9-T1048-cpGR2(#4670)
(Plasmid #250780)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250780 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5565
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCas9 with a cpGR2 insertion behind T1048
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5079
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyCas9-T1048-cpGR2(#4670) was a gift from Dominik Niopek (Addgene plasmid # 250780 ; http://n2t.net/addgene:250780 ; RRID:Addgene_250780)

  • For your References section:

    Modular Engineering of Thermo-Responsive Allosteric Proteins. Kroell AS, Hoffmann KH, Motzkus NA, Lemmen N, Happ N, Wolf B, von Bachmann A, Southern N, Vogd F, Aschenbrenner S, Niopek D, Mathony J. Nat Chem Biol (2026). 10.1038/s41589-026-02151-y
Related items: