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CAA705
(Plasmid #250781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250781 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEX2T
  • Backbone manufacturer
    Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 pLysS cells for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TOP2B C-terminal domain
  • Alt name
    3820-4866 (stop codon)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1046
  • Entrez Gene
    TOP2B (a.k.a. BILU, TOPIIB, top2beta)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAA705 was a gift from Caroline Austin (Addgene plasmid # 250781 ; http://n2t.net/addgene:250781 ; RRID:Addgene_250781)
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