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pAAV-CAGIG-Malat_Barcode_101
(Plasmid #250804)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250804 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    CAGIG (addgene #222394)
  • Backbone manufacturer
    Pantelis Tsoulfas
  • Backbone size w/o insert (bp) 759
  • Total vector size (bp) 4831
  • Modifications to backbone
    The WPRE was removed to facilitate nuclear retention
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Malat_Barcode_101
  • Species
    H. sapiens (human)
  • GenBank ID
    NR_002819.5
  • Promoter CAG or CAGIG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCACGGS-5
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAGIG-Malat_Barcode_101 was a gift from Murray Blackmore (Addgene plasmid # 250804 ; http://n2t.net/addgene:250804 ; RRID:Addgene_250804)