pAAV-CAGIG-Malat_Barcode_103
(Plasmid
#250806)
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PurposeExpresses a molecular barcode sequence attached to the nuclear localization sequence of the lncRNA Malat for enhanced detection in single nucleus sequencing libraries
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneCAGIG (addgene #222394)
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Backbone manufacturerPantelis Tsoulfas
- Backbone size w/o insert (bp) 4071
- Total vector size (bp) 4830
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Modifications to backboneThe WPRE was removed to facilitate nuclear retention
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMalat_Barcode_103
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SpeciesH. sapiens (human)
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GenBank IDNR_002819.5
- Promoter CAG or CAGIG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCACGGS-5
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAGIG-Malat_Barcode_103 was a gift from Murray Blackmore (Addgene plasmid # 250806 ; http://n2t.net/addgene:250806 ; RRID:Addgene_250806)