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pLJM1-CRS-Halo-RNaseL
(Plasmid #250857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250857 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLJM1-puro
  • Backbone size w/o insert (bp) 7345
  • Total vector size (bp) 10894
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNASEL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    363
  • Entrez Gene
    POLR2M (a.k.a. GCOM1, GRINL1A, Gdown, Gdown1)
  • Entrez Gene
    RNASEL (a.k.a. PRCA1, RNS4)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • GDOWN1(aa 250-368)-HaloTag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1-CRS-Halo-RNaseL was a gift from Britt Glaunsinger (Addgene plasmid # 250857 ; http://n2t.net/addgene:250857 ; RRID:Addgene_250857)
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