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pDUAL-pAct1-Sp_IntAct-tADH1
(Plasmid #250862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250862 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDUAL-Pact1-MCS-terADH1
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2, URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sp_IntAct
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1173
  • GenBank ID
    NM_001021513.2 Y00447.1
  • Promoter TEF
  • Tag / Fusion Protein
    • ALFA tag

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDUAL-pAct1-Sp_IntAct-tADH1 was a gift from Saravanan Palani (Addgene plasmid # 250862 ; http://n2t.net/addgene:250862 ; RRID:Addgene_250862)

  • For your References section:

    IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms. van Zwam MC, Dhar A, Bosman W, van Straaten W, Weijers S, Seta E, Joosten B, van Haren J, Palani S, van den Dries K. PLoS Biol. 2024 Mar 11;22(3):e3002551. doi: 10.1371/journal.pbio.3002551. eCollection 2024 Mar. 10.1371/journal.pbio.3002551 PubMed 38466773
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