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pLVX Neo TagBFP-LC3B
(Plasmid #250866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250866 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LC3B
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tag / Fusion Protein
    • TagBFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CMVF
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX Neo TagBFP-LC3B was a gift from Thomas Melia (Addgene plasmid # 250866 ; http://n2t.net/addgene:250866 ; RRID:Addgene_250866)

  • For your References section:

    ATG2A engages RAB1A and ARFGAP1 positive membranes during autophagosome biogenesis. Fuller DM, Wu Y, Schueder F, Rasool B, Nag S, Korfhage JL, Garcia-Milian R, Melnyk KD, Bewersdorf J, De Camilli P, Melia TJ. eLife 2025 14:RP107316 10.7554/eLife.107316.1
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