pM2_PCTR1EGFP_PJ23102R1LuxR
(Plasmid
#250885)
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PurposeEGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepZ
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEGFP
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SpeciesBacteria
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Insert Size (bp)1914
- Promoter PCT
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
- 3′ cloning site Unspecified (unknown if destroyed)
- 5′ sequencing primer Unspecified
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameLuxR
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SpeciesBacteria
- Promoter BBa_J23102
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
- 3′ cloning site Unspecified (unknown if destroyed)
- 5′ sequencing primer Unspecified
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM2_PCTR1EGFP_PJ23102R1LuxR was a gift from Sangram Bagh (Addgene plasmid # 250885 ; http://n2t.net/addgene:250885 ; RRID:Addgene_250885) -
For your References section:
Multicellular artificial neural network-type architectures demonstrate computational problem solving. Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S. Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 10.1038/s41589-024-01711-4. Epub 2024 Sep 16. 10.1038/s41589-024-01711-4 PubMed 39285005