pK1_PHT_R4_EGFP
(Plasmid
#250889)
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PurposeEGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepZ
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesBacteria
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Insert Size (bp)736
- Promoter PHT
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK1_PHT_R4_EGFP was a gift from Sangram Bagh (Addgene plasmid # 250889 ; http://n2t.net/addgene:250889 ; RRID:Addgene_250889) -
For your References section:
Multicellular artificial neural network-type architectures demonstrate computational problem solving. Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S. Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 10.1038/s41589-024-01711-4. Epub 2024 Sep 16. 10.1038/s41589-024-01711-4 PubMed 39285005