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pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR
(Plasmid #250890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250890 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pZ
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    E2-Crimson
  • Species
    Bacteria
  • Insert Size (bp)
    3735
  • Promoter PLC

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)
  • 3′ cloning site Unspecified (unknown if destroyed)
  • 5′ sequencing primer Unspecified
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    LuxR
  • Promoter BBa_J23102

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)
  • 3′ cloning site Unspecified (unknown if destroyed)
  • 5′ sequencing primer Unspecified
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Mutated cI
  • Mutation
    Frame shifted cI protein
  • Promoter PLTetO1

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)
  • 3′ cloning site Unspecified (unknown if destroyed)
  • 5′ sequencing primer Unspecified
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    Mutated cI
  • Mutation
    Frame shifted cI protein, point mutation in PLux
  • Promoter Plux

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)
  • 3′ cloning site Unspecified (unknown if destroyed)
  • 5′ sequencing primer Unspecified
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR was a gift from Sangram Bagh (Addgene plasmid # 250890 ; http://n2t.net/addgene:250890 ; RRID:Addgene_250890)

  • For your References section:

    Multicellular artificial neural network-type architectures demonstrate computational problem solving. Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S. Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 10.1038/s41589-024-01711-4. Epub 2024 Sep 16. 10.1038/s41589-024-01711-4 PubMed 39285005
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