pM2_PLLacO1_R1_mTagBFP2
(Plasmid
#250898)
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PurposemTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepZ
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2
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SpeciesBacteria
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Insert Size (bp)723
- Promoter PL LacO1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene's sequencing results differ from the partial sequence information provided by the depositing laboratory by mTagBFP2 N-terminal amino acids "MS" instead of the expected "MVSKGE". The depositor confirms that this change is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM2_PLLacO1_R1_mTagBFP2 was a gift from Sangram Bagh (Addgene plasmid # 250898 ; http://n2t.net/addgene:250898 ; RRID:Addgene_250898) -
For your References section:
Multicellular artificial neural network-type architectures demonstrate computational problem solving. Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S. Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 10.1038/s41589-024-01711-4. Epub 2024 Sep 16. 10.1038/s41589-024-01711-4 PubMed 39285005