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pSBbi-cpla2-mKate2-P2A-EGFP-KDEL
(Plasmid #250904)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250904 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 5678
  • Total vector size (bp) 9585
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cPla2-mKate2-P2A-EGFP-KDEL
  • Alt name
    phospholipase A2, group IVAa
  • Alt name
    cPla2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3907
  • Entrez Gene
    pla2g4aa (a.k.a. PLA2G4A, cpla2, pla2g4)
  • Promoter EF1-alpha
  • Tags / Fusion Proteins
    • mKate2 on cPla2 (C terminal on insert)
    • KDEL on EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsu15I (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer gtttgtacaaaaaagcaggc (ATTB1)
  • 3′ sequencing primer tagaaggcacagtcgagg (BGHR)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBbi-cpla2-mKate2-P2A-EGFP-KDEL was a gift from Philipp Niethammer (Addgene plasmid # 250904 ; http://n2t.net/addgene:250904 ; RRID:Addgene_250904)

  • For your References section:

    Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction. Shen Z, Gelashvili Z, Niethammer P. Nat Cell Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9. 10.1038/s41556-025-01820-9 PubMed 41366519
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