SNAIL-Luc
(Plasmid #250936)

• Purpose: Lentiviral vector encoding a truncated promoter region of human SNAIL gene driving a luciferase reporter
• Depositing Lab: Michelle Farkas
• Publication: Chhe et al Clocks Sleep. 2025 Sep 28;7(4):54. doi: 10.3390/clockssleep7040054.

Backbone

• Vector backbone: pMA3160
• Backbone manufacturer: Alexeyev Lab
• Backbone size w/o insert (bp): 8331
• Total vector size (bp): 8737
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SNAIL promoter
• Alt name: SNAI1
• Species: H. sapiens (human)
• Insert Size (bp): 340
• Mutation: Contains a 340 bp region of the truncated SNAIL promoter
• GenBank ID: 6615
• Entrez Gene: SNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
• Promoter: SNAIL

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CCG GAA TTC AGG TGA CCC GCC TCT TAA
• 3′ sequencing primer: ATA AGA ATG CGG CCG CGG GCG GGG CCT TAT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: See information in comments.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For generation of SNAIL-luciferase (SNAIL-Luc) plasmid, a 769bp EcoRI/NotI fragment was isolated from a pGL2 basic SNAIL construct, obtained from Addgene (plasmid #31694, deposited by Dr Paul Wade). The SNAIL promoter-containing fragment was subcloned into the lentiviral construct pMA3160 (Addgene plasmid #35043, deposited by Dr Mikhail Alexeyev) to generate a SNAIL-luciferase reporter construct.