hPER2.2-luc
(Plasmid #250937)

• Purpose: Lentiviral vector encoding a truncated promoter region of human PER2 gene driving a luciferase reporter
• Depositing Lab: Michelle Farkas
• Publication: Kalyanaraman et al Int J Mol Sci. 2025 Nov 6;26(21):10785. doi: 10.3390/ijms262110785.

Backbone

• Vector backbone: pMA3160
• Backbone manufacturer: Alexeyev Lab
• Backbone size w/o insert (bp): 8331
• Total vector size (bp): 9288
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PERIOD 2 Promoter
• Alt name: PER2
• Species: H. sapiens (human)
• Insert Size (bp): 952
• Mutation: contains 952 bp of the truncated human PER2 promoter
• Entrez Gene: PER2 (a.k.a. FASPS, FASPS1)
• Promoter: PER2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ccggaattcAGGTGGAGGTCTCCCTCGTCCGGCT
• 3′ sequencing primer: AAATATgcggccgcGGAGGGTTCCCAAAAGAGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: See comments.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For generation of PER2-luciferase (PER2-Luc) plasmid, a 952-bp EcoRI/NotI fragment was isolated from a pGL3 basic PER2 construct, obtained from Dr. Helmut Zarbl (Rutgers University). The PER2 promoter-containing fragment was subcloned into the lentiviral construct pMA3160 (Addgene plasmid #35043, deposited by Dr Mikhail Alexeyev) to generate a PER2-luciferase reporter construct.