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pKC148_Lenti_Tetoff_MVP_EF1a_tagRFP
(Plasmid #250949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250949 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
  • Backbone manufacturer
    Emma Rawlins (Addgene plasmid # 167935)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    tagRFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MVP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2679
  • Entrez Gene
    MVP (a.k.a. LRP, VAULT1)
  • Promoter TRE3G

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thomas Muir (Addgene plasmid # 202206)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKC148_Lenti_Tetoff_MVP_EF1a_tagRFP was a gift from Fei Chen (Addgene plasmid # 250949 ; http://n2t.net/addgene:250949 ; RRID:Addgene_250949)

  • For your References section:

    A genetically encoded device for transcriptome storage in mammalian cells. Chao YK, Wu M, Gong Q, Chen F. Science. 2026 Jan 15:eadz9353. doi: 10.1126/science.adz9353. 10.1126/science.adz9353 PubMed 41538410
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