pFSD
(Plasmid
#250975)
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Purpose(Empty Backbone) Minimal replicative empty plasmid for Shewanella sp.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJRD215
- Backbone size (bp) 5278
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Modifications to backboneConjugation elements and Sm resistance genes removed. MCS introduced.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFSD was a gift from Peter Sarin (Addgene plasmid # 250975 ; http://n2t.net/addgene:250975 ; RRID:Addgene_250975) -
For your References section:
Queuosine promotes wecB-dependent phage resistance and biofilm formation in marine bacterium Shewanella glacialimarina. Gregorova P, Heinonen MMK, Sipari N, Sarin LP. bioRxiv 2026.03.05.709803 10.64898/2026.03.05.709803