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pUCminiTn7-Mariner-sfC-nlp1
(Plasmid #250981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250981 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC18 miniTn7-lac
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid is propagated under ampiciillin selection in E. coli. It is non replicative in Pseudomonas aeruginosa, but inserts a gentamicin cassette along with genes of interest into the Tn7 site in the Pseudomonas chromosome.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mariner transposase fused to sfCherry and phage protein Nlp1

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUCminiTn7-Mariner-sfC-nlp1 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 250981 ; http://n2t.net/addgene:250981 ; RRID:Addgene_250981)
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