pHERD20T-V3TnAcrVIA1
(Plasmid
#250984)
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PurposepHERD20T vector providing the transposon donor with acrVIA1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepHERD20T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSelection in Pseudomonas aeruginosa requires ~250µg/mL of carbenicillin. Ampicillin is used for propagation in E. coli.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTransposon donor V3 encoding acrVIA1
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHERD20T-V3TnAcrVIA1 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 250984 ; http://n2t.net/addgene:250984 ; RRID:Addgene_250984)