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rEstus2s
(Plasmid #250991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250991 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1(+)_Puro
  • Backbone size w/o insert (bp) 5142
  • Total vector size (bp) 6423
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rEstus2s
  • Species
    G. gallus (chicken); A. victoria
  • Insert Size (bp)
    1281
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer TAG AAG GCA CAG TCG AGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.02.10.701308 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rEstus2s was a gift from Stefan Heinemann (Addgene plasmid # 250991 ; http://n2t.net/addgene:250991 ; RRID:Addgene_250991)

  • For your References section:

    Sub-Millivolt Voltage Imaging Reveals Gap Junction-Mediated Bioelectric Contact Inhibition. Rühl P, Hussein R, Reuter S, Frahnert K, Nair AG, Mrowka R, Schönherr R, Heinemann SH. bioRxiv 2026.02.10.701308; doi: 10.64898/2026.02.10.701308 10.64898/2026.02.10.701308
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