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pAH_DEST_nHalo-Sec61β-3XFLAG
(Plasmid #250996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250996 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAH_DEST_nHalo-3XFLAG
  • Backbone manufacturer
    DNASU
  • Total vector size (bp) 6035
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEC61β
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tags / Fusion Proteins
    • Halo Tag (N terminal on backbone)
    • 3XFLAG (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The vector backbone was originally acquired from DNASU (cat no: EvNO00983632) and is under a MTA with the University of Iowa.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAH_DEST_nHalo-Sec61β-3XFLAG was a gift from Anthony Pedley (Addgene plasmid # 250996 ; http://n2t.net/addgene:250996 ; RRID:Addgene_250996)

  • For your References section:

    Coordination of cell organelles to promote metabolon formation. Sha Z, Pedley AM, Iles TD, Zhang S, Staub JR, Zhou R, Benkovic SJ. Proc Natl Acad Sci U S A. 2026 Feb 3;123(5):e2532504123. doi: 10.1073/pnas.2532504123. Epub 2026 Jan 26. 10.1073/pnas.2532504123 PubMed 41587318
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