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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])
(Plasmid #251108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251108 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggyBac
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Mutation
    N/A
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Albert W. Cheng

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Originally described in Taghbalout et al., 2019 (https://doi.org/10.1038/s41467-019-12339-7/)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS]) was a gift from Chia-Lin Wei (Addgene plasmid # 251108 ; http://n2t.net/addgene:251108 ; RRID:Addgene_251108)

  • For your References section:

    Extrachromosomal DNA associates with nuclear condensates and reorganizes chromatin structures to enhance oncogenic transcription. Taghbalout A, Tung CH, Clow PA, Tjong H, Wang P, Wong CH, Mao DD, Maurya R, Huang MF, Ngan CY, Kim AH, Wei CL. Cancer Cell. 2025 Dec 8;43(12):2191-2205.e6. doi: 10.1016/j.ccell.2025.08.008. Epub 2025 Sep 18. 10.1016/j.ccell.2025.08.008 PubMed 40972571
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