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pAT3159-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank4_COLO320 DM_ AT_sg-297-303])
(Plasmid #251116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251116 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    piggyBac
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNAs
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Mutation
    N/A

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT3159-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank4_COLO320 DM_ AT_sg-297-303]) was a gift from Chia-Lin Wei (Addgene plasmid # 251116 ; http://n2t.net/addgene:251116 ; RRID:Addgene_251116)

  • For your References section:

    Extrachromosomal DNA associates with nuclear condensates and reorganizes chromatin structures to enhance oncogenic transcription. Taghbalout A, Tung CH, Clow PA, Tjong H, Wang P, Wong CH, Mao DD, Maurya R, Huang MF, Ngan CY, Kim AH, Wei CL. Cancer Cell. 2025 Dec 8;43(12):2191-2205.e6. doi: 10.1016/j.ccell.2025.08.008. Epub 2025 Sep 18. 10.1016/j.ccell.2025.08.008 PubMed 40972571
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