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TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
(Plasmid #251173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251173 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Minimalistic Amp + ColE1 backbone
  • Backbone size w/o insert (bp) 1969
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5503
  • Entrez Gene
    LGR5 (a.k.a. FEX, GPR49, GPR67, GRP49, HG38)
  • Tag / Fusion Protein
    • ires-mNeon-nls-p2a-dtr (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Puromycin selection cassette is flanked by LoxP sites for later removal. Please visit https://doi.org/10.1101/2025.07.13.664589 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro was a gift from Hugo Snippert (Addgene plasmid # 251173 ; http://n2t.net/addgene:251173 ; RRID:Addgene_251173)
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