TV_EMPTY-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
(Plasmid
#251174)
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PurposeEmpty targeting vector (SapI sites intact) for C-terminal knockin at desired locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 251174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneMinimalistic Amp + ColE1 backbone
- Backbone size w/o insert (bp) 1969
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedouble SapI flanked ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
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SpeciesSynthetic
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Insert Size (bp)4617
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Tag
/ Fusion Protein
- ires-mNeon-nls-p2a-dtr (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Puromycin selection cassette is flanked by LoxP sites for later removal. Please visit https://doi.org/10.1101/2025.07.13.664589 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TV_EMPTY-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro was a gift from Hugo Snippert (Addgene plasmid # 251174 ; http://n2t.net/addgene:251174 ; RRID:Addgene_251174)
