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pVG173
(Plasmid #251208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251208 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    EZL105
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EL222 codon-optimized for budding yeast
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Gly80Arg
  • Promoter PGK1pr

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVG173 was a gift from Sahand Jamal Rahi (Addgene plasmid # 251208 ; http://n2t.net/addgene:251208 ; RRID:Addgene_251208)

  • For your References section:

    Light-directed evolution of dynamic, multi-state, and computational protein functionalities. Gligorovski V, Labagnara M, Scutteri L, Blackholm M, Moglich A, Mansouri N, Rahi SJ. Cell. 2026 Mar 6:S0092-8674(26)00167-4. doi: 10.1016/j.cell.2026.02.002. 10.1016/j.cell.2026.02.002 PubMed 41794024
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